Call 800-808-0490 for Quote. Fluoresentric's design team develops your real-time PCR assay to perform as a duplex (two target) reaction with optimal reaction sensitivity. Your assay is designed to peform optimally under a single set of reaction conditions with two targets co-amplifying. Following the completion of the development cycle, your assay is delivered as a custom read-to-use assay. Fluoresentric's assay design staff will design the assay you need. Then we actually test it. The assay will detect your target specifically or you don't pay. Included with each package is the following: Design Details - includes oligo sequences Confirmed optimized thermal cycling conditions 200 reactions of oligonucleotides 2 hours of customer support Please call 800-808-0490 for a custom quote. PCR Assay Development by Fluoresentric.