Detection of the scrapie predisposing alleles at codon 136 of the PRNP gene in Ovis aries.
Detects in a single tube each of the possible alleles: GCC (Ala); GTC (Val)
Designed with Roche SimpleProbe chemistry using the fluorescein chemistry. Can be used for genotyping on any real-time detection instrument that is capable of detecting fluorescein signals and also perform a melting curve analysis following the amplification of product.
100 reactions of 20uL volumes of pre-tested and qc/qa verified oligonucleotide sets.
Add water and use the included buffers the FluorEnhance 10x PCR buffer (FI-RTPB-01), 25mM Magnesium chloride mix (FI-MC-01), Molecular Biology Grade Water (FI-MBW-01) with a exonuclease (-) thermostable DNA polymerase. Contact Fluoresentric for recommendations.