Detection of the scrapie predisposing alleles at codon 154 of the PRNP gene in Ovis aries. Detects in a single tube each of the possible alleles: CGT (Arg); GAC (His) Designed with Roche SimpleProbe chemistry using the fluorescein chemistry. Can be used for genotyping on any real-time detection instrument that is capable of detecting fluorescein signals and also perform a melting curve analysis following the amplification of product. 200 reactions of 20uL volumes of pre-tested and qc/qa verified oligonucleotide sets. Add water and use the included FluorEnhance 10x PCR buffer (FI-RTPB-01), 25mM Magnesium chloride mix (FI-MC-01), Molecular Biology Grade Water (FI-MBW-01) with a exonuclease (-) thermostable DNA polymerase. Contact Fluoresentric for recommendations.
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