Fluoresentric, using our proprietary design method, will design a reasonable primer set and determine the actual sequence of the target that you want to detect. Service is based on a per target fee. Real-time PCR applications require exquisite knowledge of the target sequence that you mean to detect. If you want to perform real-time PCR with an undocumented sequence. . . let Fluoresentric determine the right sequence for you from your target. Fluoresentric will provide between 150 and 300bp of sequence information for your project. Enough for you or Fluoresentric to design a suitable real-time PCR detection assay. PCR Assay Design by Fluoresentric.